Batch correction after TMM or non-normalized

[yeswzc]

Hi, in the workflow, you did normalization and get an object of spe_tmm from spe.
Batch correction was later performed by spe not spe_tmm.
Should I use spe to perform batch correction or spe_tmm?

Thanks

[ningbioinfo]

Hi, sorry for the late reply. But either way, it shouldn't affect the DE results much.